Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine
Identifieur interne : 003B63 ( Main/Exploration ); précédent : 003B62; suivant : 003B64Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine
Auteurs : Alexander N. Zakhartchouk [Canada] ; Chetna Sharon [Canada] ; Malathy Satkunarajah [Canada] ; Thierry Auperin [Canada] ; Sathiyanarayanan Viswanathan [Canada] ; George Mutwiri [Canada] ; Martin Petric [Canada] ; Raymond H. See [Canada] ; Robert C. Brunham [Canada] ; B. Brett Finlay [Canada] ; Cheryl Cameron [Canada] ; David J. Kelvin [Canada] ; Alan Cochrane [Canada] ; James M. Rini [Canada] ; Lorne A. Babiuk [Canada]Source :
- Vaccine [ 0264-410X ] ; 2007.
Descripteurs français
- KwdFr :
- Alun, Animaux, Anticorps antiviraux (sang), Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Interféron gamma (biosynthèse), Lignée cellulaire, Oligodésoxyribonucléotides, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Souris, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux.
- biosynthèse : Interféron gamma.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux.
- sang : Anticorps antiviraux.
- Pascal (Inist)
- Alun, Animaux, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Oligodésoxyribonucléotides, Protéines de l'enveloppe virale, Souris, Syndrome respiratoire aigu sévère, Virus syndrome respiratoire aigu sévère, Souris, Immunogénicité, Protéine, Sousunité, Vaccin.
- Wicri :
- topic : Vaccin.
English descriptors
- KwdEn :
- Alum Compounds, Animals, Antibodies, Viral (blood), Cell Line, Female, Humans, Immunogenicity, Interferon-gamma (biosynthesis), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mouse, Oligodeoxyribonucleotides, Protein, Receptors, Virus (metabolism), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe acute respiratory syndrome virus, Spike Glycoprotein, Coronavirus, Subunit, Vaccine, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , biosynthesis : Interferon-gamma.
- chemical , blood : Antibodies, Viral.
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , metabolism : Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemical : Alum Compounds, Oligodeoxyribonucleotides, Spike Glycoprotein, Coronavirus.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Female, Humans, Mice.
Abstract
We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-γ-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-γ-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.
Affiliations:
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Le document en format XML
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type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement 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aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Mutwiri, George" sort="Mutwiri, George" uniqKey="Mutwiri G" first="George" last="Mutwiri">George Mutwiri</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road</s1><s2>Saskatoon, Sask., S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>University of British Columbia Centre for Disease Control</s1><s2>Vancouver, BC, V5Z 4R4</s2><s3>CAN</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>University of British Columbia Centre for Disease Control</wicri:noRegion></affiliation></author><author><name sortKey="See, Raymond H" sort="See, Raymond H" uniqKey="See R" first="Raymond H." last="See">Raymond H. See</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>University of British Columbia Centre for Disease Control</s1><s2>Vancouver, BC, V5Z 4R4</s2><s3>CAN</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>University of British Columbia Centre for Disease Control</wicri:noRegion></affiliation></author><author><name sortKey="Brunham, Robert C" sort="Brunham, Robert C" uniqKey="Brunham R" first="Robert C." last="Brunham">Robert C. Brunham</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>University of British Columbia Centre for Disease Control</s1><s2>Vancouver, BC, V5Z 4R4</s2><s3>CAN</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>University of British Columbia Centre for Disease Control</wicri:noRegion></affiliation></author><author><name sortKey="Finlay, B Brett" sort="Finlay, B Brett" uniqKey="Finlay B" first="B. Brett" last="Finlay">B. Brett Finlay</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Michael Smith Laboratories and Departments of Biochemistry and Molecular Biology and Microbiology and Immunology, University of British Columbia</s1><s2>Vancouver, BC, V6T 1Z3</s2><s3>CAN</s3><sZ>10 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Vancouver, BC, V6T 1Z3</wicri:noRegion></affiliation></author><author><name sortKey="Cameron, Cheryl" sort="Cameron, Cheryl" uniqKey="Cameron C" first="Cheryl" last="Cameron">Cheryl Cameron</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Experimental Therapeutics, University Health Network, Toronto General Research Institute</s1><s2>Toronto, Ont</s2><s3>CAN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Toronto, Ont</wicri:noRegion></affiliation></author><author><name sortKey="Kelvin, David J" sort="Kelvin, David J" uniqKey="Kelvin D" first="David J." last="Kelvin">David J. Kelvin</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Experimental Therapeutics, University Health Network, Toronto General Research Institute</s1><s2>Toronto, Ont</s2><s3>CAN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Toronto, Ont</wicri:noRegion></affiliation></author><author><name sortKey="Cochrane, Alan" sort="Cochrane, Alan" uniqKey="Cochrane A" first="Alan" last="Cochrane">Alan Cochrane</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M." last="Rini">James M. Rini</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A." last="Babiuk">Lorne A. Babiuk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road</s1><s2>Saskatoon, Sask., S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0078280</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0078280 INIST</idno><idno type="RBID">Pascal:07-0078280</idno><idno type="wicri:Area/PascalFrancis/Corpus">000418</idno><idno type="wicri:Area/PascalFrancis/Curation">000571</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000374</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000374</idno><idno type="wicri:doubleKey">0264-410X:2007:Zakhartchouk A:immunogenicity:of:a</idno><idno type="wicri:Area/Main/Merge">003D21</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:16919855</idno><idno type="wicri:Area/PubMed/Corpus">002118</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002118</idno><idno type="wicri:Area/PubMed/Curation">002118</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002118</idno><idno type="wicri:Area/PubMed/Checkpoint">001D84</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001D84</idno><idno type="wicri:Area/Ncbi/Merge">001630</idno><idno type="wicri:Area/Ncbi/Curation">001630</idno><idno type="wicri:Area/Ncbi/Checkpoint">001630</idno><idno type="wicri:doubleKey">0264-410X:2007:Zakhartchouk A:immunogenicity:of:a</idno><idno type="wicri:Area/Main/Merge">003820</idno><idno type="wicri:Area/Main/Curation">003B63</idno><idno type="wicri:Area/Main/Exploration">003B63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Immunogenicity of a receptor-binding domain of SARS coronavirus spike protein in mice : Implications for a subunit vaccine</title><author><name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N." last="Zakhartchouk">Alexander N. Zakhartchouk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road</s1><s2>Saskatoon, Sask., S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road</s1><s2>Saskatoon, Sask., S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Mutwiri, George" sort="Mutwiri, George" uniqKey="Mutwiri G" first="George" last="Mutwiri">George Mutwiri</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road</s1><s2>Saskatoon, Sask., S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>University of British Columbia Centre for Disease Control</s1><s2>Vancouver, BC, V5Z 4R4</s2><s3>CAN</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>University of British Columbia Centre for Disease Control</wicri:noRegion></affiliation></author><author><name sortKey="See, Raymond H" sort="See, Raymond H" uniqKey="See R" first="Raymond H." last="See">Raymond H. See</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>University of British Columbia Centre for Disease Control</s1><s2>Vancouver, BC, V5Z 4R4</s2><s3>CAN</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>University of British Columbia Centre for Disease Control</wicri:noRegion></affiliation></author><author><name sortKey="Brunham, Robert C" sort="Brunham, Robert C" uniqKey="Brunham R" first="Robert C." last="Brunham">Robert C. Brunham</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>University of British Columbia Centre for Disease Control</s1><s2>Vancouver, BC, V5Z 4R4</s2><s3>CAN</s3><sZ>7 aut.</sZ><sZ>8 aut.</sZ><sZ>9 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>University of British Columbia Centre for Disease Control</wicri:noRegion></affiliation></author><author><name sortKey="Finlay, B Brett" sort="Finlay, B Brett" uniqKey="Finlay B" first="B. Brett" last="Finlay">B. Brett Finlay</name><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>Michael Smith Laboratories and Departments of Biochemistry and Molecular Biology and Microbiology and Immunology, University of British Columbia</s1><s2>Vancouver, BC, V6T 1Z3</s2><s3>CAN</s3><sZ>10 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Vancouver, BC, V6T 1Z3</wicri:noRegion></affiliation></author><author><name sortKey="Cameron, Cheryl" sort="Cameron, Cheryl" uniqKey="Cameron C" first="Cheryl" last="Cameron">Cheryl Cameron</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Experimental Therapeutics, University Health Network, Toronto General Research Institute</s1><s2>Toronto, Ont</s2><s3>CAN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Toronto, Ont</wicri:noRegion></affiliation></author><author><name sortKey="Kelvin, David J" sort="Kelvin, David J" uniqKey="Kelvin D" first="David J." last="Kelvin">David J. Kelvin</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Division of Experimental Therapeutics, University Health Network, Toronto General Research Institute</s1><s2>Toronto, Ont</s2><s3>CAN</s3><sZ>11 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Toronto, Ont</wicri:noRegion></affiliation></author><author><name sortKey="Cochrane, Alan" sort="Cochrane, Alan" uniqKey="Cochrane A" first="Alan" last="Cochrane">Alan Cochrane</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M." last="Rini">James M. Rini</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Departments of Molecular and Medical Genetics and Microbiology, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>13 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation><affiliation wicri:level="4"><inist:fA14 i1="03"><s1>Department of Biochemistry, University of Toronto</s1><s2>Ont., M5S 1A8</s2><s3>CAN</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName><orgName type="university">Université de Toronto</orgName></affiliation></author><author><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A." last="Babiuk">Lorne A. Babiuk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, 120 Veterinary Road</s1><s2>Saskatoon, Sask., S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>5 aut.</sZ><sZ>6 aut.</sZ><sZ>15 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, Sask., S7N 5E3</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Vaccine</title><title level="j" type="abbreviated">Vaccine</title><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alum Compounds</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Cell Line</term><term>Female</term><term>Humans</term><term>Immunogenicity</term><term>Interferon-gamma (biosynthesis)</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (immunology)</term><term>Membrane Glycoproteins (metabolism)</term><term>Mice</term><term>Mouse</term><term>Oligodeoxyribonucleotides</term><term>Protein</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe acute respiratory syndrome virus</term><term>Spike Glycoprotein, Coronavirus</term><term>Subunit</term><term>Vaccine</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viral Vaccines (administration & dosage)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alun</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (immunologie)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Interféron gamma (biosynthèse)</term><term>Lignée cellulaire</term><term>Oligodésoxyribonucléotides</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Souris</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Vaccins antiviraux (administration et posologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Alum Compounds</term><term>Oligodeoxyribonucleotides</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron gamma</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Female</term><term>Humans</term><term>Mice</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Alun</term><term>Animaux</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Lignée cellulaire</term><term>Oligodésoxyribonucléotides</term><term>Protéines de l'enveloppe virale</term><term>Souris</term><term>Syndrome respiratoire aigu sévère</term><term>Virus syndrome respiratoire aigu sévère</term><term>Souris</term><term>Immunogénicité</term><term>Protéine</term><term>Sousunité</term><term>Vaccin</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Vaccin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We studied the immunogenicity of an anti-SARS subunit vaccine comprised of the fragment of the SARS coronavirus (SARS-CoV) spike protein amino acids 318-510 (S318-510) containing the receptor-binding domain. The S protein fragment was purified from the culture supernatant of stably transformed HEK293T cells secreting a tagged version of the protein. The vaccine was given subcutaneously to 129S6/SvEv mice in saline, with alum adjuvant or with alum plus CpG oligodeoxynucleotides (ODN). Mice immunized with the adjuvanted antigen elicited strong antibody and cellular immune responses; furthermore, adding the CpG ODN to the alum resulted in increased IgG2a antibody titers and a higher number of INF-γ-secreting murine splenocytes. Mice vaccinated with S318-510 deglycosylated by PNGase F (dgS318-510) showed a lower neutralizing antibody response but had similar numbers of INF-γ-producing cells in the spleen. This finding suggests that carbohydrate is important for the immunogenicity of the S318-510 protein fragment and provide useful information for designing an effective and safe SARS subunit vaccine.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><country name="Canada"><noRegion><name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N." last="Zakhartchouk">Alexander N. Zakhartchouk</name></noRegion><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name><name sortKey="Auperin, Thierry" sort="Auperin, Thierry" uniqKey="Auperin T" first="Thierry" last="Auperin">Thierry Auperin</name><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A." last="Babiuk">Lorne A. Babiuk</name><name sortKey="Brunham, Robert C" sort="Brunham, Robert C" uniqKey="Brunham R" first="Robert C." last="Brunham">Robert C. Brunham</name><name sortKey="Cameron, Cheryl" sort="Cameron, Cheryl" uniqKey="Cameron C" first="Cheryl" last="Cameron">Cheryl Cameron</name><name sortKey="Cochrane, Alan" sort="Cochrane, Alan" uniqKey="Cochrane A" first="Alan" last="Cochrane">Alan Cochrane</name><name sortKey="Finlay, B Brett" sort="Finlay, B Brett" uniqKey="Finlay B" first="B. Brett" last="Finlay">B. Brett Finlay</name><name sortKey="Kelvin, David J" sort="Kelvin, David J" uniqKey="Kelvin D" first="David J." last="Kelvin">David J. Kelvin</name><name sortKey="Mutwiri, George" sort="Mutwiri, George" uniqKey="Mutwiri G" first="George" last="Mutwiri">George Mutwiri</name><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name><name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M." last="Rini">James M. Rini</name><name sortKey="Rini, James M" sort="Rini, James M" uniqKey="Rini J" first="James M." last="Rini">James M. Rini</name><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name><name sortKey="Satkunarajah, Malathy" sort="Satkunarajah, Malathy" uniqKey="Satkunarajah M" first="Malathy" last="Satkunarajah">Malathy Satkunarajah</name><name sortKey="See, Raymond H" sort="See, Raymond H" uniqKey="See R" first="Raymond H." last="See">Raymond H. See</name><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name><name sortKey="Sharon, Chetna" sort="Sharon, Chetna" uniqKey="Sharon C" first="Chetna" last="Sharon">Chetna Sharon</name><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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